1. Banyere nrụpụta nke akpa nje
A virus sampling tubes bụ ngwaahịa Ngwaọrụ. Edebere ọtụtụ ndị na-emepụta ụlọ dị ka ngwaahịa ndị mbụ na klas, na ụlọ ọrụ ole na ole dị ka ngwaahịa nke abụọ. N'oge na-adịbeghị anya, iji mezuo mkpa ihe mberede nke WUHN na ebe ndị ọzọ, ọtụtụ ụlọ ọrụ na-ese foto "na-enweghị ụkpụrụ nchekwa obodo ma ọ bụ ọkọlọtọ nke ndị na-emepụta mba dị iche iche.
1 A ga-eme nke swab swab nke polyester (pe) synhetic eriri ma ọ bụ raken (eriri mmadụ mere). Agaghị enwe ike iji sponge ma ọ bụ osisi swabs (gụnyere osisi achara), na ihe nke swab isi agaghị abụ ngwaahịa. N'ihi na eriri owu nwere protein siri ike, ọ naghị adịrị gị mfe na ngwọta nchekwa ahụ; Ma mgbe osisi achara ma ọ bụ ramboo nwere calcinate na osisi osisi ga-agbaji, na-eme ka protein protein ga-agbaji, ọ ga-emekwa ka ọ na-egbochi mmeghachi omume Pcr na-esote. A na-atụ aro iji eriri eji sịntetik dịka fiber, polyester eriri na eriri polypropylene maka ihe nke swab isi. A naghị atụ aro ka anụ arụ dị ka akwa dị ka owu. A na-akwakwa eriri nylon n'ihi na nylon eriri (nke yiri isi ezé) na-abanye mmiri. Ogbenye, nke butere usoro nlele ezughi oke, na-emetụta ọnụego nchọpụta ahụ. Amachibidoro ihe nlegharị anya calvge nke ọma maka ihe nlele nke swab! Swab nwere ụdị abụọ: agbajiri ma wuru. A na-etinye swab gbajiri agbaji na nchekwa nchekwa mgbe satpling, na eriri tube mgbe etinyere ya n'ọnọdụ dị nso na isi iyi ahụ dị nso; Swab na swab swab na-etinye swab swab na-etinye na nchekwa nchekwa mgbe nlele, a na-ewukwa tube tube na elu nke aka ahụ na-emechi mkpuchi ahụ. Ikwuru ụzọ abụọ ahụ, nke ikpeazụ ahụ dịtụ nchebe. Mgbe ejiri swab gbajiri agbaji na obere nchekwa nha, ọ nwere ike ibute mmiri mmiri na tube ahụ mgbe agbajiri agbaji na ihe ọghọm nke iji ngwaahịa a na-adịghị mma. A na-atụ aro ka eji polystyrene (ps) exuded tube ma ọ bụ polypropylene (PP) na-atọ ụtọ maka ihe nke swab. N'agbanyeghị ihe a na-eji, ihe mgbakwunye calcium algetive; osisi ma ọ bụ osisi achara. Na nkenke, sampling swab kwesịrị iji hụ na ị ga - akwụ ụgwọ, yana ihe ndị ahọpụtara agaghị enwe ihe ndị metụtara ule ndị ahụ.
2. Ngwọta Ngwọta Nje Virus: E nwere ụdị nchekwa nchekwa virus nke na-akpatakarị n'ahịa, otu bụ ihe mgbakwunye na-arụ ọrụ na-agbanwe dabere na ndị na-ebugharị ebugharị, na nke ọzọ bụ ngwọta acid mmịpụta nke ngụgụ nucleic.
Ihe bụ isi nke onye bụbu Eagle ọdịiche nke ọdịbendị ọdịnala ma ọ bụ nke na-edozi ahụ, nke na-agbakwunye na nnu, vitamin, glucose na protein dị mkpa maka ịlanarị nje virus. Ngwọta nchekwa a na-eji Sọnol Red Sink dị ka onye na-egosi na ngwọta. Mgbe uru pH dị 6.6-8.0, ihe ngwọta ya bụ pink. A na-etinye glucose dị mkpa, l-glutamine na protein na-agbakwunye na ngwọta nchekwa. A na-enye protein n'ụdị fetal Bovine ma ọ bụ Bovine album album, nke nwere ike ime ka protein shei nke nje ahụ. N'ihi na nchekwa nchekwa bara ụba na nri, ọ na-enyere gị aka ịlanarị nje ahụ kama ọ bakwara uru na nje bacteria. Ọ bụrụ na njehie nchekwa ahụ merụrụ ahụ, ọ ga-aba ụba n'ọtụtụ. Carbon dioxide na metabolis ya ga-eme ka nchekwa nchekwa nke ga-ada site na pink na-acha odo odo. Ya mere, ọtụtụ ndị na-emepụta ihe atụnyerela ihe ndị na-egbochi nje maka usoro ha. Ndị ọrụ ọgwụ mgbochi a tụrụ aro bụ penicillin, streptomycin, onye Jentanicy na plyylxin-3-methyzollin-3-methyzolin-3-otu (cyci) n'ihi na ihe ndị a nwere mmetụta na pcr Mmeghachi omume. Ebe ọ bụ na ihe nlereanya nke nchekwa a nyere bụ nje virus, ebe a ga-echekwa ya na ọ bụghị naanị maka mmịpụta na nchọpụta nke nje virus na nje. Agbanyeghị, ekwesịrị ịchọpụta na mgbe ejiri ya maka nchọpụta, a ga-arụ ọrụ ngụkọta nucleic na ọcha.
A na-akwado ụdị nchekwa nchekwa nke actic ọzọ dabere na nnu, Edta na-eme ihe nkiri, dị ka Guanidine Surractate), dị ka Tetraductrimethyladimethylamtylamtrium Oxalet), phenol, 8-hydroxyquine, dithiothreitol (dtt), ihe na-enye ya nri k na ihe ndị ọzọ mejupụtara ya iji hapụ nucleic acid ma kpochapụ fnase. Ọ bụrụ na ejiri naanị RT-pcr, ọ dabara adaba, mana igwe nwere ike ịta ahụhụ nje. A gaghị eji ụdị ihe nlele a maka ọdịbendị ọdịnala virus.
A na-atụ aro na ihe nrịba ama nke igwe na-atụ aro iji nnu nje EDRA (dịka sodium ethlenestiaminet acid, wdg.)
3. Ichebe tube: ihe nke tube na-ahọpụtara. Enwere data na-atụ aro na polypropylene (polypropyylene) bụ ihe a na-ekwu na ya bụ ihe kachasị amasị karịa polypropylene (polypropylene) dị mfe iji belata DNA / RNA. Polymene-propylene-propylene (polylomer) plastic polypropylene (polypropyylene (polypropylene) nke ọma dị mma maka nchekwa DNA / RNA. Na mgbakwunye, mgbe ị na-eji swab swab, tube kwesịrị ịnwa ịhọrọ akpa nwere ihe dị elu karịa 8 cm iji gbochie ihe ndị a na-emebi emebi ma mebie ya.
4 Mmiri ma ọ bụ mmiri dị iche.
2. Ojiji nke tutu uzo virus
Samppling site na iji ntụpọ virus na-ekewa na samplingngeal sampling na nasopharyngeal sampling:
1. O ma O ma OrppresingnGaryngeal: Nke mbu pịa ire ire na mgbidi pharyngeal na mgbidi pharyngeal na ike ọkụ, zere imetụ n 'ire ọkụ.
2. Na-enyocha nlele nasopharyngeal: tụọ anya site n'ọnụ ọnụ imi na mbata nke imi, na-eme ka ọ bụrụ ọkara nke imi, jiri nwayọọ na-emegharị ugboro 3-5, ma weghaara ya swab.
O sighị ike ịhụ ụzọ o si arụ ọrụ, ma ọ bụ swab swab ma ọ bụ a nasopharyngeal swab, sampling bụ ọrụ ọrụ aka, nke siri ike ma merụọ. A na-eme ka ihe nlele a na-anakọta na-emetụta ya na nyocha ndị na-esote. Ọ bụrụ n 'nlele a anakọtara nwere nsogbu dị ala, dị mfe iji mee ka nyocha ụgha, sie ike ikwenye nchọpụta ahụ.
Oge post: Jun-21-2020
